Number of curves (n):
Paste in X, Y1, Y2, Y3... Yn values:
Paste in the X and Y values here. They should be entered as a table where the first column is for the X-values and the remaining columns are for the corresponding Y-values in each of the curves. Usually any X value will be a substrate concentration and it should be followed by the corresponding experimental rate measurements (Y-values) for the separate curves in the remaining columns with intervening spaces or text separators. You must indicate how many columns of Y-values you have entered by using the drop-down menu button (above) or the table will not be read correctly! If any measurements are missing (e.g. if at a certain value of X, the values in the first and third Y-columns have been measured but that for the second Y-column has not) this should be indicated by a question mark with some spaces each side to separate out the columns clearly. There must, of course, be no question marks in the X-column! Duplicate measurements can be entered all together in the same manner. Data should be entered as ordinary decimal numbers, integers or in exponential 'E' or 'e' format. Non-numerical text should not matter since the program will try to remove it, with the exception of question marks which are interpreted as missing measurements (see above). If the plot does not appear or the curves do not fit well, please check that the number of curves is correct and that the data are entered correctly. Sometimes to get the fitting routine to work you need to scale down the X-values, e.g. by converting them all from mM to M or uM to mM, nM to uM, etc. Note that the fitting relies on the measurement at the highest value of X providing a reasonable estimate of the Y-asymptote!
Tick here and re-submit if curves look sigmoid.